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KMID : 0644120170300040001
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Journal of Oriental Gynecology
2017 Volume.30 No. 4 p.1 ~ p.17
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Effects of Hyeolbuchugeo-tang on Osteoclast Differentiation and Bone Resorption
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Jang Sae-Byul
Yoo Dong-Youl
Yoo Jeong-Eun
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Abstract
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Objectives: This study was conducted to evaluate the effects of Hyeolbuchugeo-tang (HBC) on Osteoporosis.
Methods: We induced RAW 264.7 cells to differentiate to Osteoclasts by RANKL and treated RANKL-induced RAW 264.7 cells with HBC (0, 150, 350, 700 μg/ml). To measure osteoclast differentiation and activation, we counted TRAP (+) MNCs and measured mRNA expressions of its related genes (TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF, iNOS, COX-2, TNF-α) by RT-PCR. To assess bone resorption, the Bone pit formation were examined under a microscope.
Results: HBC decreased TRAP (+) MNCs and inhibited mRNA expressions of TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF in osteoclast. And HBC inhibited Bone pit formation.
Conclusions: HBC inhibited osteoclast differentiation and activation and bone resorption. Taken together, these results indicate that HBC might have potentials for prevention and treatment of Osteoporosis.
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KEYWORD
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Hyeolbuchugeo-tang , Osteoporosis , Osteoclasts , Osteoclastogenesis , Bone Resorption , Bone Pit
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